Bax Group DC Server - Dipolar Wave Fitting for RDCs in Helix

PDB Input PDB Input Atom names in the PDB format file must exactly match names used in the RDC input file. Example: ubiq.pdb Select this option to add H atoms to the input PDB file, hydrogen atoms will be replaced if already present. [More Info] Add hydrogens to PDB
Dipolar Coupling Inputs Dipolar Coupling Inputs [At Least 2 Files Required!] Dipolar couplings measured from samples in different medium should be stored in separated input files, and uploaded by using the Files Selection option on the left (press and hold the Ctrl key to select multiple files in Windows or the Command key in MacOS) Examples: dObsA.tab (Neutral Bicelle Sample) \| dObsB.tab (Charged Bicelle Sample) \| dObsP.tab (Pf1 Sample) \| dObsS.tab (Squalamine Sample).
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About For sets of RDCs measured from N alignment media, DC first calculates the alignment tensor for each medium by SVD-fitting the RDCs to the input reference structure normalizes all N alignment tensors to a uniform alignment magnitude (Da) of 10 Hz performs a SVD operation to orthogonalize above normalized alignment tensors generates a new set of n (=min(N,5)) orthogonalized RDC datasets (stored in files dOrth1.tab, dOrth2.tab ...) [Note: orthogonalized RDC is generated only for couplings which are complete for all N alignment media] generates graphics with both the original alignment tensors relative to the molecular structure , and the orthogonalized tensors Reference: J.L. Lorieau, A.S. Maltsev, J.M. Louis, and A. Bax: Modulating alignment of membrane proteins in liquid-crystalline and oriented gel media by changing the size and charge of phospholipid bicelles. J. Biomol. NMR. 55, 369-377 (2013). (DOI) (pubmed)

	From the Bax Group at the National Institutes of Health DC: Servers for Residual Dipolar Coupling Calculations Orthogonalize Alignment Tensors Using SVD and Generate Orthogonalized RDCs