Solution structure of the ~25 kDa IIAMannitol-HPr Complex of the E. coli Phosphotransferase System

 

 

overall pts pathway

 

 

IIAMtl-HPr complex

IIAMTl-HPr complex

Backbone superposition and reweighted atomic density for selected side chains obtaine from 200 simulated annealing structures

IIAMtl-HPr complex

 

The phosphoryl transition state

transition state

 

 

 

Comparison of PTS complexes with HPr

 

comparison with other pts complexes

 

Cornilescu, G., Lee, B.R., Cornilescu, C.C., Wang, G., Peterkofsky, A. & Clore, G.M. (2002) Solution structure of the phosphoryl transfer complex between the cytoplasmic A domain of the mannitol transporter IIMannitol and HPr of the Escherichia coli phosphotransferase system. J. Biol. Chem. 277, 42289-42298. Pubmed PDF

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