Solution structure of the Anti-HIV Protein Cyanovirin-N in its Monomeric and Domain-Swapped Dimeric Forms

 

Comparison of solution structure of monomeric form of CVN with X-ray structure of the domain-swapped dimer

CVN a

Comparison of solution and X-ray structures of domain-swapped dimer
(Note that the residual dipolar couplings measured for the dimer in solution are not compatible with the relative orientations of the two halves of the domain-swapped dimer observed in the crystal structure)

CVN b

 

Bewley, C.A., Gustafson, K.R., Boyd, M.R., Covell, D.G., Bax, A., Clore, G.M. & Gronenborn, A.M. (1998) Solution structure of cyanovirin-N, a potent HIV-inactivating protein. Nature Struct. Biol. 5, 571-578. pubmed PDF

Yang, F., Bewley, C.A., Louis, J.M., Gustafson, K.R., Boyd, M.R., Gronenborn, A.M., Clore, G.M. & Wlodawer, A. (1999) Crystal structure of cyanovirin-N, a potent HIV-inactivating protein, shows unexpected domain swapping. J. Mol. Biol. 288, 403-412. pubmed PDF

Bewley, C.A. & Clore, G.M. (2000) Determination of the relative orientation of the two halves of the domain-swapped dimer of cyanovirin-N in solution using dipolar couplings and rigid body minimization. J. Am. Chem. Soc. 122, 6009-6016. PDF

Clore, G.M. & Bewley, C.A. (2002) Using conjoined rigid body / torsion angle simulated annealing to determine the relative orientation of covalently linked protein domains from dipolar couplings. J. Magn. Reson. 154, 329-335. pubmed PDF

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