• 2D IPAP [15N,1H] HSQC
• pulse code
• frequency table

• 2D IPAP HNCO
• pulse code

• 3D J-modulated HSQC
• pulse code
• gradient program


• 2D Constant-time TROSY J N-C'
  • pulse code
  
  
• 3D TROSY HNCO J N-C'
  • pulse code
  
  
• CBCA(CO)NH-JCH 3D Quantitative J Correlation
  • pulse code
  • example data and analysis software
  
  
• 2D IPAP [13C,1H] CT-HSQC for Methyl Groups
  • pulse code
  • description
  • tar archive of NMRPipe processing scripts
  
  
• 3D Long Range 1H-1H Dipolar Coupling
  • pulse code
  
  
• Long Range Dipolar Coupling with Nearest-Neighbor Decoupling
  • tar archive pulse code and NMRPipe scripts
  
  
• Measurement of RDC in nucleic acid bases HC(C)-hd-TROSY-ECOSY
  • Purine version, tar archive pulse code
  • Pyrimidine version, tar archive pulse code
  • H5C5-hd-TROSY Sample Data and NMRPipe Scripts
  • H5C5(C6)-hd-TROSY-ECOSY Sample Data and NMRPipe Scripts
  • Sample Data for intermediate processing and optimization
  • Information on processing optimization (PDF)
  
  
• 2D Selective CT 1H-31P NOESY
  • pulse code
  
  
• 3D TROSY HNCO Quantitative J C'-CA
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D TROSY HN(CO)CA Quantitative J C'-CA
  • tar archive pulse code and NMRPipe scripts
  
  
• Pulse Sequences for J(NC) in Nucleic Acids
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D spin-state selective HBCBCA
  • pulse code
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D spin-state selective H5'C5'C4'
  • pulse code
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D HNCA MQ Experiments for 3J Measurement
  • 3D CT-MQ(1HN,13CA)+SQ(1HN)-HNCA spectra for 3JHN,HA (pulse code)
  • 3D MQ(1HN,13CA)-HNCA experiments for 3JHN,CB (pulse code)
  • 3D CT-MQ(1HN,13CA)-HNCA spectrum for 3JHN,C' (pulse code)
  • tar archive pulse code, parameters and NMRPipe scripts
  
  
• 2D ARTSY for 1DNH RDC measurement
• pulse code
• zipped pulse code and example processing scripts


• CH2-TROSY HACAN
• pulse code
• zipped pulse code and example processing scripts


• Hydrogen Exchange (WEX-III) TROSY
• pulse code
• zipped pulse code and example processing scripts


• imino 15N-1H and base 13C-1H ARTSY for 1DNH and 1DCH RDC measurement in RNA
• pulse sequences, acqu parameters and example processing scripts
• Scripts and input files for structure modeling


• SJS-HSQC for small molecule RDC (Angew. Chem. 2011, 50, 1-6)
• Bruker pulse sequence, acqu and proc parameters


• ARTSY-HNCO for measuring 1DHN couplings (JACS 2021, 143, 19306-19310)
• Bruker pulse sequence and acquisition parameters as well as NMRPipe processing scripts


• TATER-HNCO for measuring 2D(HN-C') couplings (JACS 2021, 143, 19306-19310)
• Bruker pulse sequence and acquisition parameters as well as NMRPipe processing scripts


• Include File (bits.nt)

• Anisotropic Shift Measurements:
  H1C1C2
  H1C1(C2)C3
  H1C1(C2C3)C4
  (H5)C5(C4C3C2)C1H1
  
  • tar archive pulse code, parameters, and NMRPipe scripts
  
  
• H1'->C1' -> C2' Out-and-Back CCH COSY
  • tar archive pulse code and NMRPipe scripts
  
  
• CH2-TROSY Experiments:
  2D [13C,1H] Correlation for DNA
  2D [13C,1H] Correlation for Glycine
  2D [13C,1H] Correlation for Protein Sidechains
  3D [13C,1H,1H] TROSY-NOESY for DNA
  • tar archive pulse code and NMRPipe conversion scripts
  
  
• 3D constant-time parallel evolution HMQC-IPAP-NOESY
  • pulse code
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D mixed-time parallel evolution HMQC-NOESY
  • tar archive pulse code and NMRPipe scripts


• HSQC and TROSY experiments for measuring RNA base 13C residual CSA and pseudo-CSA shift
  • tar archive pulse codes, acquisition parameters and NMRPipe scripts


• 15N Relaxation Experiments (TROSY and sensitivity-enhanced HSQC) (Lakomek et al., J. Biomol. NMR 53, 209-221, 2012)
  • pulse code
  • acquisition parameters
  • nmrPipe scripts


• 1H T2 measurement by 2D Hahn-echo TROSY (Lakomek et al., Angewandte Chemie 52: 3911-3915, 2013)
  • pulse code


• Homonuclear decoupling for enhancing resolution and sensitivity in NOE and RDC measurements of peptides and proteins (Ying, J.; Roche, J.; Bax, A. (2014) J. Magn. Reson. 2014, 241, 97-102)
  • Bruker acqu parameters and pulse code for 1D BASH (analog mode)
  • Updated Bruker pulse code for 1D BASH in the digital mode
  • Bruker acqu parameters and pulse code for 2D noesy with BASH
  • Bruker acqu parameters and pulse code for HSQC with BASH
  • Bruker acqu parameters and pulse code for 3D HA coupled hncoca with BASH


• HNCOCONH for the measurement of 3J(C'C') (Li, F.; Lee, J.H.; Grishaev, A.; Ying, J.; Bax, A. ChemPhysChem, 2015; Lee, J.H.; Li, F.; Grishaev, A.; Bax, A. J. Am. Chem. Soc. 137: 1432-1435, 2015)
• tar archive pulse sequence, include file, acqu parameters, processing scripts


• Accurate Measurement of (3)JHNHa Couplings in Small or Disordered Proteins from WATERGATE-optimized TROSY Spectra Roche, J.; Ying, J.; Bax, A. J. Biomol. NMR, 2016, 64, 1-7.
• tar archive pulse sequence, acqu parameters, processing scripts


• ARTSY-J: Convenient and precise measurement of 3JHNHa couplings in mediume-size proteins from TROSY-HSQC spectra Roche, J.; Ying, J.; Shen, Y.; Torchia, D.A.; Bax, A. J. Magn. Reson., 2016, 268, 73-81.
• tar archive pulse sequence, acqu parameters, processing scripts

Standard Files:

• Include file