Dr. John L. Marquardt, Jr.

National Institutes of Health
NIDDK, LCP
Building 5, Room B1-28
9000 Rockville Pike
Bethesda, MD 20892-0520

Tel.: 301-496-7902
Fax: 301-496-0825

E-mail: marquardt@alum.mit.edu
 


RESEARCH INTERESTS

Structural Biology, Structure Determination with Nuclear Magnetic Resonance
 

EDUCATION

Ph.D. in Biochemistry and Molecular Pharmacology
Harvard University, Boston, Massachusetts, with Prof. Christopher T. Walsh, 1993
 
B.S. in Physics
Massachusetts Institute of Technology, 1988
 

PROFESSIONAL EXPERIENCE

Post-Doctoral Fellow
National Institutes of Health, Bethesda, Maryland, USA, since 1994
 

AWARDS  AND FELLOWSHIPS

I.R.T.A. Fellowship, 1995 - present
Helen Hay Whitney Foundation Fellowship, 1994 - 1997
Ryan Fellowship, 1991 - 1993
N.I.H. Training Award , 1988 - 1991
High Scholarship in Physics, SPS M.I.T. , 1988
 

PUBLICATIONS

Publications from the Bax Group

Previous  Publications:
Brown, E. D., J. L. Marquardt, J. P. Lee, C. T. Walsh, and K. S. Anderson, Detection and Characterization of a Phospholactyl-enzyme Adduct in the Reaction Catalyzed by UDP-N-AcetylGlucosamine Enolpyruvoyl Transferase, MurZ, Biochemistry, Vol. 33, 1994, p. 10638-45.

Marquardt, J. L., E. D. Brown, W. S. Lane, T. D. Haley, Y. Ichikawa, C-H Wong, and C. T. Walsh, Kinetics, Stoichiometry and Identification of the Active Site Thiol for Phosphomycin Inactivation of UDP-GlcNAc Enolpyruvate Transferase, Biochemistry, Vol. 33, 1994, p. 10646-51.

Marquardt, J. L., E. D. Brown, C. T. Walsh, and K. S. Anderson, Isolation and Structural Elucidation of a Tetrahedral Intermediate in the UDP-N-acetylglucosamine Enolpyruvoyl Transferase Enzymatic Pathway, Journal of the American Chemical Society, Vol. 115, 1993, p. 10398-9.

Benson, T. E., J. L. Marquardt, A. C. Marquardt, F. A. Etzkorn and C. T. Walsh, Overexpression, Purification and Mechanistic Study of UDP-N-Acetylenolpyruvylglucosamine Reductase, Biochemistry, Vol. 32, 1993, p. 2024-2030.

Marquardt, J. L., D. A. Siegele, R. Kolter and C. T. Walsh, Cloning and Sequencing of the E.coli murZ Gene and Purification of its Product, a UDP-N-Acetylglucosamine Enolpyruvyl Transferase, Journal of Bacteriology, Vol. 174, No. 17,  1992, p. 5748-5752.



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