Technical drawings of the funnel-like gel-stretching apparatus. It is used for radially compressing polyacrylamide gel of diameter d0 into an open-ended NMR tube of internal diameter dN, thus weakly aligning macromolecules inside the hydrogel. After compression, the aspect ratio of the gel is (d0/dN)^3.
The assembly consists of five parts, labeled 1-5:
In the technical drawing, INSTANCES A, B, C, and D correspond to apparatus
dimensions for different degrees of radial compression, yielding the
following gel aspect ratios:
Funnels and other accessories can now be purchased from NewEra Research, for information contact email@example.com
James J. Chou, Sander Gaemers, Bernard Howder, John M. Louis, and Ad Bax. "A simple apparatus for generating stretched polyacrylamide gels, yielding uniform alignment of proteins and detergent micelles" J. Biomol. NMR 21, 377-382 (2001). (302.pdf, 116030 Bytes)