;NOESY-P selective CT-NOESY interleaved for measuring H-P couplings ;added dephasing during shaped pulse ;Justin Wu and Ad bax, JBNMR, 19, 367-370 (2001) #include "Gradnt_new.incl" ; SFO2 = 242.940 for nucleic acids ; This experiment works correctly ;"d20=35.1m" "d0 = 5u" "d3=p3" "d4=p6*0.5-p3" "d18 = d20-d0" "d19=d18-p3*2" 1 ze 2 50m 3 10u ;fq1:f1 10u pl18:f1 10u cw:f1 d1 pl3:f2 10u do:f1 ; 10u fq1:f1 10u pl1:f1 p1 ph1 d20 5u pl18:f1 if "l1==1" goto 20 (p6:sp0 ph2):H (d4 p3*2 ph0):f2 10u goto 21 20 (p6:sp0 ph2):H 10u 21 10u d18 3u pl1:f1 (p1 ph11 3u p1*2 ph12 3u p1 ph11):f1 (p3*2 ph0):f2 if "l1==1" goto 10 3u (p6:sp0 ph15):H (d4 p3*2 ph0):f2 6u goto 11 10 3u (p6:sp0 ph15):H 6u 11 d0 pl1:f1 p1 ph0 d5 ;NOE mixing p1 ph3 go=2 ph31 50m wr #0 if #0 zd 10u iu1 lo to 3 times 2 10u ru1 10u ip1 lo to 3 times 2 10u id0 10u dd20 10u ip31 10u ip31 lo to 3 times l3 exit ph0=0 ph1=0 2 ph2=0 0 1 1 2 2 3 3 ph3=0 0 0 0 1 1 1 1 2 2 2 2 3 3 3 3 ph11=0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ph12=1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ph15=0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ph31=0 2 2 0 1 3 3 1 2 0 0 2 3 1 1 3