Pulse Sequence Library

This library contains recently published pulse sequences that have been tested on our equipment. By downloading, viewing or using any of them you acknowledge our rules as outlined in disclaimer and copyright.

Experiments for Measurement of Dipolar Couplings:

• 2D IPAP [15N,1H] HSQC
• pulse code
• frequency table

• 2D IPAP HNCO
• pulse code

• 3D J-modulated HSQC
• pulse code
• gradient program


• 2D Constant-time TROSY J N-C'
  • pulse code
  
  
• 3D TROSY HNCO J N-C'
  • pulse code
  
  
• CBCA(CO)NH-JCH 3D Quantitative J Correlation
  • pulse code
  • example data and analysis software
  
  
• 2D IPAP [13C,1H] CT-HSQC for Methyl Groups
  • pulse code
  • description
  • tar archive of NMRPipe processing scripts
  
  
• 3D Long Range 1H-1H Dipolar Coupling
  • pulse code
  
  
• Long Range Dipolar Coupling with Nearest-Neighbor Decoupling
  • tar archive pulse code and NMRPipe scripts
  
  
• Measurement of RDC in nucleic acid bases HC(C)-hd-TROSY-ECOSY
  • Purine version, tar archive pulse code
  • Pyrimidine version, tar archive pulse code
  • H5C5-hd-TROSY Sample Data and NMRPipe Scripts
  • H5C5(C6)-hd-TROSY-ECOSY Sample Data and NMRPipe Scripts
  • Sample Data for intermediate processing and optimization
  • Information on processing optimization (PDF)
  
  
• 2D Selective CT 1H-31P NOESY
  • pulse code
  
  
• 3D TROSY HNCO Quantitative J C'-CA
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D TROSY HN(CO)CA Quantitative J C'-CA
  • tar archive pulse code and NMRPipe scripts
  
  
• Pulse Sequences for J(NC) in Nucleic Acids
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D spin-state selective HBCBCA
  • pulse code
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D spin-state selective H5'C5'C4'
  • pulse code
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D HNCA MQ Experiments for 3J Measurement
  • 3D CT-MQ(1HN,13CA)+SQ(1HN)-HNCA spectra for 3JHN,HA (pulse code)
  • 3D MQ(1HN,13CA)-HNCA experiments for 3JHN,CB (pulse code)
  • 3D CT-MQ(1HN,13CA)-HNCA spectrum for 3JHN,C' (pulse code)
  • tar archive pulse code, parameters and NMRPipe scripts
  
  
• 3D HNCA gradient enhanced
• to be released


• Include File (bits.nt)

Other Experiments:

• Anisotropic Shift Measurements:
  H1C1C2
  H1C1(C2)C3
  H1C1(C2C3)C4
  (H5)C5(C4C3C2)C1H1
  
  • tar archive pulse code, parameters, and NMRPipe scripts
  
  
• H1'->C1' -> C2' Out-and-Back CCH COSY
  • tar archive pulse code and NMRPipe scripts
  
  
• CH2-TROSY Experiments:
  2D [13C,1H] Correlation for DNA
  2D [13C,1H] Correlation for Glycine
  2D [13C,1H] Correlation for Protein Sidechains
  3D [13C,1H,1H] TROSY-NOESY for DNA
  • tar archive pulse code and NMRPipe conversion scripts
  
  
• 3D constant-time parallel evolution HMQC-IPAP-NOESY
  • pulse code
  • tar archive pulse code and NMRPipe scripts
  
  
• 3D mixed-time parallel evolution HMQC-NOESY
  • tar archive pulse code and NMRPipe scripts


• HSQC and TROSY experiments for measuring RNA base 13C residual CSA and pseudo-CSA shift
  • tar archive pulse codes, acquisition parameters and NMRPipe scripts

Standard Files:

• Include file