Ad Bax Group / NMR Pulse Programs

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Bax Group - Pulse Programs

This library contains pulse sequences used and published in our group.
By downloading, viewing or using any of them you are agreeing to the Terms of Use.

Experiments for Measurement of Dipolar Couplings:

2D IPAP [15N,1H] HSQC

2D IPAP HNCO

pulse code

3D J-modulated HSQC

2D Constant-time TROSY J N-C'
- pulse code
3D TROSY HNCO J N-C'
- pulse code
CBCA(CO)NH-JCH 3D Quantitative J Correlation
- pulse code
- example data and analysis software
2D IPAP [13C,1H] CT-HSQC for Methyl Groups
- pulse code
- description
- tar archive of NMRPipe processing scripts
3D Long Range 1H-1H Dipolar Coupling
- pulse code
Long Range Dipolar Coupling with Nearest-Neighbor Decoupling
- tar archive pulse code and NMRPipe scripts
Measurement of RDC in nucleic acid bases HC(C)-hd-TROSY-ECOSY
- Purine version, tar archive pulse code
- Pyrimidine version, tar archive pulse code
- H5C5-hd-TROSY Sample Data and NMRPipe Scripts
- H5C5(C6)-hd-TROSY-ECOSY Sample Data and NMRPipe Scripts
- Sample Data for intermediate processing and optimization
- Information on processing optimization (PDF)
2D Selective CT 1H-31P NOESY
- pulse code
3D TROSY HNCO Quantitative J C'-CA
- tar archive pulse code and NMRPipe scripts
3D TROSY HN(CO)CA Quantitative J C'-CA
- tar archive pulse code and NMRPipe scripts
Pulse Sequences for J(NC) in Nucleic Acids
- tar archive pulse code and NMRPipe scripts
3D spin-state selective HBCBCA
- pulse code
- tar archive pulse code and NMRPipe scripts
3D spin-state selective H5'C5'C4'
- pulse code
- tar archive pulse code and NMRPipe scripts
3D HNCA MQ Experiments for 3J Measurement
- 3D CT-MQ(1HN,13CA)+SQ(1HN)-HNCA spectra for 3JHN,HA (pulse code)
- 3D MQ(1HN,13CA)-HNCA experiments for 3JHN,CB (pulse code)
- 3D CT-MQ(1HN,13CA)-HNCA spectrum for 3JHN,C' (pulse code)
- tar archive pulse code, parameters and NMRPipe scripts
2D ARTSY for 1DNH RDC measurement

CH2-TROSY HACAN

Hydrogen Exchange (WEX-III) TROSY

imino 15N-1H and base 13C-1H ARTSY for 1DNH and 1DCH RDC measurement in RNA

SJS-HSQC for small molecule RDC (Angew. Chem. 2011, 50, 1-6)

Bruker pulse sequence, acqu and proc parameters

ARTSY-HNCO for measuring 1DHN couplings (JACS 2021, 143, 19306-19310)

Bruker pulse sequence and acquisition parameters as well as NMRPipe processing scripts

TATER-HNCO for measuring 2D(HN-C') couplings (JACS 2021, 143, 19306-19310)

Bruker pulse sequence and acquisition parameters as well as NMRPipe processing scripts

Include File (bits.nt)

Other Experiments:

Anisotropic Shift Measurements:
H1C1C2
H1C1(C2)C3
H1C1(C2C3)C4
(H5)C5(C4C3C2)C1H1
- tar archive pulse code, parameters, and NMRPipe scripts
H1'->C1' -> C2' Out-and-Back CCH COSY
- tar archive pulse code and NMRPipe scripts
CH2-TROSY Experiments:
2D [13C,1H] Correlation for DNA
2D [13C,1H] Correlation for Glycine
2D [13C,1H] Correlation for Protein Sidechains
3D [13C,1H,1H] TROSY-NOESY for DNA
- tar archive pulse code and NMRPipe conversion scripts
3D constant-time parallel evolution HMQC-IPAP-NOESY
- pulse code
- tar archive pulse code and NMRPipe scripts
3D mixed-time parallel evolution HMQC-NOESY
- tar archive pulse code and NMRPipe scripts

HSQC and TROSY experiments for measuring RNA base 13C residual CSA and pseudo-CSA shift
- tar archive pulse codes, acquisition parameters and NMRPipe scripts

15N Relaxation Experiments (TROSY and sensitivity-enhanced HSQC) (Lakomek et al., J. Biomol. NMR 53, 209-221, 2012)

1H T2 measurement by 2D Hahn-echo TROSY (Lakomek et al., Angewandte Chemie 52: 3911-3915, 2013)
- pulse code

Homonuclear decoupling for enhancing resolution and sensitivity in NOE and RDC measurements of peptides and proteins (Ying, J.; Roche, J.; Bax, A. (2014) J. Magn. Reson. 2014, 241, 97-102)

HNCOCONH for the measurement of 3J(C'C') (Li, F.; Lee, J.H.; Grishaev, A.; Ying, J.; Bax, A. ChemPhysChem, 2015; Lee, J.H.; Li, F.; Grishaev, A.; Bax, A. J. Am. Chem. Soc. 137: 1432-1435, 2015)

tar archive pulse sequence, include file, acqu parameters, processing scripts

Accurate Measurement of (3)JHNHa Couplings in Small or Disordered Proteins from WATERGATE-optimized TROSY Spectra Roche, J.; Ying, J.; Bax, A. J. Biomol. NMR, 2016, 64, 1-7.

tar archive pulse sequence, acqu parameters, processing scripts

ARTSY-J: Convenient and precise measurement of 3JHNHa couplings in mediume-size proteins from TROSY-HSQC spectra Roche, J.; Ying, J.; Shen, Y.; Torchia, D.A.; Bax, A. J. Magn. Reson., 2016, 268, 73-81.

tar archive pulse sequence, acqu parameters, processing scripts

Standard Files:

Include file

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last updated: Mar 10 2016 / sy